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bio-alignment-pairwise - Perform pairwise sequence alignment

Align two DNA, RNA, or protein sequences, score similarity, and identify global or local matches using Biopython.

Tags

Updated: 2026-10-16
bioinformaticssequence alignmentDNARNAproteinBiopython

Capabilities

Align two sequencesConfigure alignment scoringSelect global or local modesRetrieve optimal alignments

Typical Inputs

Two DNA, RNA, or protein sequencesAlignment modeScoring parameters

Typical Outputs

Alignment objectsAlignment scoresAligned sequences

What this skill does

  • Align two sequences
  • Configure alignment scoring
  • Select global or local modes
  • Retrieve optimal alignments
  • Calculate alignment scores
  • Extract alignment coordinates
  • Count identities and gaps

Inputs

  • Two DNA, RNA, or protein sequences
  • Alignment mode
  • Scoring parameters
  • Substitution matrix

Outputs

  • Alignment objects
  • Alignment scores
  • Aligned sequences
  • Alignment coordinates
  • Identity and gap counts
  • Percent identity

Requirements

  • Python environment
  • Biopython 1.83 or later
  • Bio.Align module

Source

  • Spec: SKILL.md

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