bio-basecalling - Convert Nanopore signals to nucleotide sequences
Convert raw Nanopore signal data to nucleotide sequences using Dorado basecaller
Tags
Updated: 2026-03-23Capabilities
Typical Inputs
Typical Outputs
What this skill does
- convert signal to sequences
- select basecalling model
- detect modified bases
- filter sequences by quality
- generate QC reports
- convert FAST5 to POD5
- trim adapters
- demultiplex samples
- perform duplex calling
- list available models
Inputs
- FAST5 files
- POD5 files
- basecalling model
- GPU device
- CPU threads
- barcode kit name
Outputs
- BAM file
- FASTQ file
- filtered sequences
- QC reports
- sequencing statistics
- sequencing summary
Requirements
- Dorado basecaller
- GPU with CUDA support
- CPU support
- POD5 tools
- Chopper or NanoFilt
- NanoPlot or pycoQC