bio-basecalling - Nanopore Basecalling with Dorado
Convert raw Nanopore signal data to nucleotide sequences using Dorado basecaller
Tags
Updated: 2026-03-23Capabilities
Typical Inputs
Typical Outputs
What this skill does
- convert FAST5 to POD5
- merge POD5 files
- inspect POD5 reads
- subset POD5 files
- run basecalling
- select Dorado model
- detect modified bases
- configure GPU device
- set batch size
- run duplex calling
- demultiplex reads
- trim adapters
- filter sequences by quality
- generate QC reports
Inputs
- FAST5 files
- POD5 files
- Dorado model
- GPU device
- batch size
- kit name
Outputs
- BAM file
- FASTQ file
- modified base calls
- filtered sequences
- QC report
- demultiplexed files
Requirements
- Dorado
- CUDA-capable GPU
- command line interface