bio-primer-design-primer-basics - Design and rank PCR primer pairs
Designs and ranks PCR primer pairs for a supplied template using primer3-py, reporting thermodynamic, sequence, size, and complementarity metrics.
Tags
Updated: 2026-10-16Capabilities
Typical Inputs
Typical Outputs
What this skill does
- Design PCR primer pairs
- Rank candidate primer pairs
- Match primer melting temperatures
- Constrain primer positions
- Report primer metrics
- Diagnose zero-pair results
Inputs
- Template sequence
- Target region
- Primer constraints
- Product-size range
- Reaction salt conditions
- Primer3 arguments
Outputs
- Ranked primer pairs
- Primer melting temperatures
- Primer GC percentages
- Product sizes
- Complementarity scores
- Pair penalties
- Zero-pair diagnostics
Requirements
- Python environment
- primer3-py 2.3 or newer
- Accessible primer3-py API
- Compatible primer3-py signatures