bio-primer-design-qpcr-primers - qPCR Primer and Probe Design
Design qPCR primers and TaqMan/molecular beacon probes using primer3-py
Tags
Updated: 2026-03-26Capabilities
Typical Inputs
Typical Outputs
What this skill does
- design qPCR primers
- design TaqMan probes
- design molecular beacon probes
- design SYBR Green primers
- design exon-spanning primers
- design multiplex primers
- calculate melting temperature
- validate Tm calculations
- format qPCR results
Inputs
- DNA sequence
- target region
- exon junction position
- Tm range
- GC content range
- product size range
- salt concentration
- dNTP concentration
- primer concentration
Outputs
- forward primer sequence
- reverse primer sequence
- probe sequence
- melting temperature
- product size
- primer-probe set data
- results dataframe
Requirements
- Python runtime
- primer3-py library
- Biopython library