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bio-single-cell-clustering - Single-Cell RNA-Seq Clustering Analysis

Perform dimensionality reduction and clustering on single-cell RNA-seq data using Seurat or Scanpy

Tags

Updated: 2026-03-20

Capabilities

Typical Inputs

Typical Outputs

What this skill does

  • Run PCA
  • Compute neighbors
  • Run Leiden clustering
  • Run Louvain clustering
  • Generate UMAP embeddings
  • Generate tSNE embeddings
  • Compute PAGA

Inputs

  • Single-cell RNA-seq data
  • AnnData object
  • Seurat object
  • n_pcs
  • n_neighbors
  • resolution

Outputs

  • Cluster labels
  • PCA coordinates
  • UMAP coordinates
  • tSNE coordinates
  • Cluster plots

Requirements

  • R with Seurat
  • Python with Scanpy

Source

  • Spec: SKILL.md

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single-cell
clustering
bioinformatics
dimensionality-reduction
scRNA-seq
Run PCA
Compute neighbors
Run Leiden clustering
Run Louvain clustering
Single-cell RNA-seq data
AnnData object
Seurat object
Cluster labels
PCA coordinates
UMAP coordinates