bio-workflows-crispr-editing-pipeline - Design complete CRISPR editing experiments
Orchestrates guide RNA design, off-target assessment, and strategy-specific construct design for knockout, base editing, HDR knock-in, or prime editing.
Tags
Updated: 2026-09-30Capabilities
What this skill does
- Design guide RNAs
- Assess off-target effects
- Design knockout strategies
- Design base editing strategies
- Design HDR knock-ins
- Design prime editing strategies
- Generate validation primers
Inputs
- Target gene or position
- Target sequence
- Reference genome FASTA
- Editing strategy
Outputs
- Guide RNA designs
- Activity and specificity scores
- Off-target assessment results
- Editing strategy designs
- Delivery-ready constructs
- Validation primers
Requirements
- Python environment
- BioPython 1.83+
- matplotlib 3.8+
- numpy 1.26+
- pandas 2.2+
- primer3-py 2.0+
- CRISPRscan
- Cas-OFFinder CLI