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Proteomics - Label-free quantitative proteomics analysis toolkit

Analyzes label-free quantitative proteomics data using R scripts for normalization, visualization, pathway analysis and protein list cross-referencing

Tags

Updated: 2026-02-11

Capabilities

Typical Inputs

Typical Outputs

What this skill does

  • normalize protein data
  • create volcano plots
  • generate heatmaps
  • run PCA analysis
  • perform pathway enrichment
  • query protein lists
  • create Excel reports
  • analyze matrisome proteins
  • identify SASP factors

Inputs

  • protein intensity data
  • sample metadata
  • comparison results
  • q-value threshold
  • fold-change cutoff
  • organism specification
  • reference protein lists
  • R scripts directory
  • project working directory

Outputs

  • TIFF plot files
  • Excel report tables
  • pathway analysis results
  • protein list matches
  • normalized data files
  • sample correlation plots
  • volcano plot images
  • heatmap visualizations

Requirements

  • R environment installed
  • specific directory structure
  • reference data files available
  • project working directory set
  • data/ subdirectory with input files
  • output/ subdirectory for results
  • Proteomics skill directory accessible

Source

  • Spec: SKILL.md

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proteomics
bioinformatics
data analysis
R scripts
quantitative analysis
pathway enrichment
protein quantification
biological markers
normalize protein data
create volcano plots
generate heatmaps
run PCA analysis
protein intensity data
sample metadata
comparison results
TIFF plot files
Excel report tables
pathway analysis results