bio-longread-qc - Long-Read Sequencing Quality Control
Quality control for long-read sequencing data using NanoPlot, NanoStat, and chopper
Tags
Updated: 2026-03-21Capabilities
Typical Inputs
Typical Outputs
What this skill does
- generate QC reports
- filter reads by quality
- filter reads by length
- visualize read characteristics
- calculate sequencing statistics
- trim adapters
- parse FASTQ quality
Inputs
- FASTQ file
- BAM file
- sequencing summary file
- quality threshold
- length threshold
- thread count
Outputs
- QC statistics file
- QC report HTML
- length vs quality plot
- read length histogram
- cumulative yield plot
- filtered FASTQ file
- trimmed FASTQ file
Requirements
- BioPython 1.83+
- numpy 1.26+
- NanoPlot
- NanoStat
- chopper
- Python 3