bio-longread-qc - Long-read sequencing data quality control
Perform quality control on long-read sequencing data using NanoPlot, NanoStat, and chopper
Tags
Updated: 2026-03-21Capabilities
Typical Inputs
Typical Outputs
What this skill does
- read FASTQ files
- read BAM files
- read sequencing summary
- filter reads by length
- filter reads by quality
- trim read adapters
- generate quality plots
- generate length distributions
- calculate summary statistics
- calculate N50
- generate QC reports
- export filtered reads
Inputs
- FASTQ files
- BAM files
- sequencing summary files
- quality thresholds
- length thresholds
Outputs
- QC report HTML files
- PNG plots
- PDF plots
- statistics text files
- filtered FASTQ files
Requirements
- Python 3.x
- BioPython 1.83+
- numpy 1.26+
- NanoPlot
- NanoStat
- chopper