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bio-metagenomics-functional-profiling - Metagenomic Functional Profiling with HUMAnN3

Profile functional potential of metagenomic samples using HUMAnN3 to get pathway and gene family abundances

Tags

Updated: 2026-03-20

Capabilities

Typical Inputs

Typical Outputs

What this skill does

  • analyze metagenomic samples
  • calculate pathway abundances
  • count gene families
  • normalize abundance data
  • regroup gene families
  • split stratified tables
  • generate quality reports
  • visualize pathway data
  • process multiple samples
  • join sample tables

Inputs

  • metagenomic fastq files
  • taxonomic profiles
  • ChocoPhlAn database
  • UniRef database
  • metadata files

Outputs

  • gene family abundance tables
  • pathway abundance tables
  • pathway coverage tables
  • normalized abundance tables
  • regrouped gene tables
  • quality control reports
  • pathway heatmaps
  • batch processing results

Requirements

  • conda environment
  • HUMAnN3 installed
  • ChocoPhlAn database
  • UniRef database

Source

  • Spec: SKILL.md

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metagenomics
functional profiling
HUMAnN3
pathway analysis
gene families
bioinformatics
analyze metagenomic samples
calculate pathway abundances
count gene families
normalize abundance data
metagenomic fastq files
taxonomic profiles
ChocoPhlAn database
gene family abundance tables
pathway abundance tables
pathway coverage tables